AEEA (CAS 134978-97-5) – Hydrophilic C6 Linker for Peptide Synthesis & Bioconjugation
2026.11.11
AEEA (2-(2-(2-Aminoethoxy)ethoxy)acetic acid) is a highly water-soluble, mini-PEG-like C6 spacer widely used in modern drug discovery. It serves as an essential building block to enhance the aqueous solubility of peptide analogs, small molecule conjugates, and therapeutics, effectively minimizing molecular aggregation. We supply AEEA in its free amine form, as well as the high-purity Fmoc-AEEA (CAS 166108-71-0), which is fully optimized for direct integration into automated solid-phase peptide synthesis (SPPS).
Technical Specifications
- CAS Number: 134978-97-5
- Purity: ≥98% (HPLC)
- Quality Control: Batch-validated by ¹H-NMR, HPLC, and Mass Spectrometry (MS)
- Chemical Formula: C6H13NO4
- Molecular Weight: 163.17 g/mol
- Appearance: Colorless to pale yellow viscous liquid / semi-solid (depending on ambient temperature)
- Storage Conditions: Store at -20°C, kept tightly sealed and protected from moisture and light
Physicochemical Properties
Unlike conventional hydrophobic aliphatic carbon chains, the ether-based backbone of AEEA imparts outstanding hydrophilic properties to target conjugates.
| Property | Value | Notes |
|---|---|---|
| Solubility | Highly soluble in water & polar organic solvents | Ideal for direct preparation in biological buffers; eliminates the need for harsh co-solvents like DMSO. |
| LogP | -1.2 | High hydrophilicity effectively screens hydrophobic patches and prevents peptide aggregation. |
| Fmoc-Protected Form | CAS 166108-71-0 | Ready-to-use crystalline solid or powder tailored for automated SPPS synthesis lines. |
Application Scenarios: Is AEEA Right for Your Project?
Recommended for:
- Long-Acting Peptide Agonists: AEEA is a standard hydrophilic side-chain extender utilized to optimize the pharmacokinetic (PK) profile, increase plasma half-life, and boost the solubility of therapeutic peptides—such as those found in blockbuster GLP-1 receptor agonist platforms .
- Solid-Phase Peptide Synthesis (SPPS): Our Fmoc-AEEA derivative features high stability during coupling and is smoothly deprotected using standard piperidine protocols without destabilizing the core polyether backbone.
- Payload Solubilization in Bioconjugates: Perfect for masking the inherent hydrophobicity of complex linker-payload structures or fluorescent dyes in diagnostic and therapeutic conjugates.
Consider Alternatives If:
- You require high plasma stability in systemic circulation: For specific non-cleavable Antibody-Drug Conjugates (ADCs), consider switching to a robust, purely hydrophobic spacer like our C14 Alkyl Chain Linker (CAS 843666-27-3).
- You need an extended spatial reach: If alleviating heavy steric hindrance around a bulky protein binding pocket is required, compare this with our AEEA-AEEA (Hydrophilic C12 Linker, CAS 1143516-05-5) for broader spatial flexibility.
Supply & Custom Synthesis
Shandong Juntai Pharmaceutical supplies AEEA and Fmoc-AEEA across scales ranging from milligrams for early-stage screening to multi-kilogram bulk quantities for commercial scale-up pipelines. Every shipment is accompanied by a batch-specific Certificate of Analysis (COA).
- Lead Time: In stock, globally dispatched within 24 hours.
- Custom Customization: Custom derivatives, salts, or multi-unit PEG linkers are available upon request through our dedicated CDMO services.
Request a Bulk Quote for CAS 134978-97-5
Frequently Asked Questions
Q: Can Fmoc-AEEA be seamlessly integrated into automated synthesizers?
A: Yes. Fmoc-AEEA (CAS 166108-71-0) is specifically engineered as a free-flowing solid or powder compatible with standard Fmoc/tBu automated chemistry, ensuring high coupling efficiency and zero recipe modifications.
Q: What are the optimal dissolution guidelines for AEEA?
A: Due to its high hydrophilicity, the free form dissolves instantly in water, PBS, and polar organic solvents like DMF or DMSO. For the Fmoc-protected form, dissolution in DMF or NMP prior to coupling is highly recommended.
Q: Are these products suitable for clinical or human trials?
A: No. All chemical linkers and intermediates supplied by our facility are strictly for Research and Development purposes (Research Use Only, RUO) and are not intended for diagnostic or therapeutic use in humans.
(All products are for Research Use Only. Not for diagnostic or therapeutic procedures.)
